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Document Type |
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Thesis |
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Document Title |
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RELATION BETWEEN VITAMIN D , CELLULAR SENESCENCE AND OBESITY TO SUBCLINICAL ATHEROSCLEROSIS AND ENDOTHELIAL DYSFUNTION العلاقة بين الفيتامين د ,الشيخوخة الخلوية والسمنة على تصلب الشرايين التحت اكلينيكي وخلل الخلايا البطانية |
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Subject |
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Faculty of medicine |
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Document Language |
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Arabic |
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Abstract |
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Background and aim of the work: The exact link between obesity, vitamin D deficiency and their relation to cellular senescence in the pathogenesis of subclinical atherosclerosis is still under debates so, the current study aims to verify the previously reported link between vitamin D status and obesity, to study the possible contribution of cellular senescence and finally to investigate the possible protective role of vitamin D supplementation against obesity-related subclinical atherosclerosis.
Material and Methods: 57 male albino rats were enrolled in the study and classified into 4 groups: negative and positive control groups (10 each), non-treated obese model group (24) and Vitamin D-treated obese group (13). Body Mass Index (BMI) was calculated weekly. Fasting blood samples were withdrawn and sera were prepared, then stored at -80°C until the time of the following biochemical investigations were performed: Serum cholesterol, triglycerides, HDL-C and LDL-C, ALT, AST, CPK, CK-MB and High sensitive Troponin T(hs-cTnt). HOMA-IR was calculated. Moreover, serum SMP-30, 25 (OH)Vitamin D, and eNOS were determined by ELISA technique.
Results:Serum 25(OH) D3 and SMP-30 are lower in obese in comparison to the other group. Serum lipid profile and HOMA-IR are higher in obese group versus control groups and decreased level is observed in vitamin D treated obese group. eNOS and cTnT are higher in obese group than the control groups and decreased level is found in vitamin D treated obese group. Positive correlation was found between vitamin D level and SMP-30 in all studied groups. |
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Supervisor |
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PROF. AYMAN ZAKY ELSAMANOUDY |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1442 AH
2021 AD |
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Co-Supervisor |
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DR. ABDULHADI IBRAHIM BIMA |
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Added Date |
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Wednesday, July 7, 2021 |
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Researchers
| عبدالله صديق مهدي | MAHDI, ABDULLAH SIDDIQ | Researcher | Master | |
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