Document Details
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Document Type |
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Thesis |
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Document Title |
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REPARATIVE CAPACITY OF RECOMBINANT AMELOGENIN PROTEIN WHEN USED AS PULP CAPPING AGENT: AN ANIMAL STUDY القابلية التجددية للمستخلص البروتيني لمادة المينا المصنع بالهندسة الوراثية (الأميلوجنين) عند استخدامه كغطاء للب : دراسة حيوانية |
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Subject |
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Faculty of Dentistry |
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Document Language |
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Arabic |
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Abstract |
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Aim of the study:
To assess the regenerative capacity of Amelogenin protein when it used as a capping agent in comparison to MTA and Bioceramic.
Material and Methods:
120 (N=120)dogs’ teeth obtained from 8-mongrel-dogs, (n=60) half of the teeth were used to evaluate the regenerative change after 2-weeks, and the other half for 2-months (n=60), White ProRoot MTA (n=40), Pre-mixed Bioceramic putty (n=40), and recombinant Amelogenin protein (n=40), were utilized as capping materials. Cavities were prepared, followed by pulp exposures and application of capping materials, after dog’s scarification the maxillary and mandibular jaws were obtained then sectioned regarding to the tooth position for the purpose of the histological analysis, serial sections were obtained and analyzed using a light microscope associated with the digital camera, the regenerative changes indicated by measuring the dentin bridge formation, the inflammatory reaction, and the pulp status.
Results:
At 2-weeks postoperatively lack of complete dentin bridge formation was the predominant feature in all groups, moderately formed dentin bridge was significantly more in the Amelogenin group compared to both groups, MTA resulted in more slight dentin bridge formation compared to Bioceramic but the difference is insignificant, the mild inflammatory reaction was observed in MTA and Amelogenin samples without a significant difference, moderate inflammatory reaction was significantly noticed in Bioceramic compared to the other capping agents. The whole pulp chamber was completely filled with pulp tissue only in Amelogenin group. The presence of dilated lymphatic vessels was significantly observed only in Bioceramic group. At 2-months follow-up period, significant increase of dentin bridge formation was observed in all the groups. Complete dentin bridge formation was significantly more in Amelogenin group. The lack of inflammatory reaction was observed in all the groups without signficant differences, the dilated blood vessels, empty pulp spaces and the presence of voids within the pulp were significantly more in MTA and Bioceramic samples without a significant difference, filling the whole pulp chamber with normal pulp tissue, lack of empty pulp spaces and voids were significantly observed more in Amelogenin group.
Conclusion:
Direct pulp capping procedure by the mean of Recombinant Amelogenin protein resulted in significantly better regeneration of the dentin-pulp complex compared to MTA and Bioceramic. |
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Supervisor |
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Dr. Laila Bahamam |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1440 AH
2019 AD |
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Co-Supervisor |
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Prof. Maha Mounier |
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Added Date |
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Monday, September 16, 2019 |
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Researchers
| وليد خالد الشرقاوي | Al Shargawi, Waleed Khaled | Researcher | Master | |
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