Main Page
About Science
Faculty Deanship
Letter of Dean
Overview of Deanship
Vice Deans
Vice Dean
Letter of Vice-dean
Overview of Vice-deanship
Vice Dean for Graduate Studies
Letter of Vice Dean for Graduate Studies
Overview of Vice Dean of Postgraduate Studies
Research and Innovation Unit
Vice Dean for Girls Campus
Faculty Management
Letter of Managing Director-Boys Campus
Letter of Managing Director-Girls Campus
Overview of Management
Educational Affairs
Males Campus
Staff
Females Campus
Contact Us
Research
عربي
English
About
Admission
Academic
Research and Innovations
University Life
E-Services
Search
Faculty of Sciences
Document Details
Document Type
:
Article In Journal
Document Title
:
Purification and characterization of acido-thermophilic xylanase from aspergillus terrus
Purification and characterization of acido-thermophilic xylanase from aspergillus terrus
Subject
:
Biological
Document Language
:
English
Abstract
:
Purification and characterization of extracellular xylanase from Aspergillus terrus was recorded. The enzyme was purified to homogeneity by salting out with ammonium sulphate, dialysis and passage through gel chromatography resins (Sephadex G-200, Sephadex G-100 columns) followed by anion exchange chromatography (Diethylaminoethyl Sephadex column). The purified enzyme resulted in 516.4 fold increase over the crude extract exhibited a specific activity of 175.6 unit/mg protein with the recovery of 30.6 %. Two criteria for the purity of the purified A. terrus extracellular xylanase were used. DEAE-sephadex column (final stage of purification) resulted in a single sharp peak of A. terrus pure xylanase. The second criterion was given by applying SDS-PAGE electrophoresis technique. The molecular weight of A. terrus extracellular xylanase was 33 KDa. Studying factors affecting the activity of the purified xylanase were determined. An optimum temperature and pH for the acidothermophilic purified xylanase were 50 °C at pH 4, respectively.
ISSN
:
1991-8178
Journal Name
:
Australian Journal of Basic and Applied Sciences
Volume
:
5
Issue Number
:
10
Publishing Year
:
1432 AH
2011 AD
Article Type
:
Article
Added Date
:
Monday, April 23, 2012
Researchers
Researcher Name (Arabic)
Researcher Name (English)
Researcher Type
Dr Grade
Email
نيفين صالح إبراهيم جويلي
Geweely, Neveen S I
Researcher
Doctorate
ngeweely@yahoo.com
Files
File Name
Type
Description
33015.pdf
pdf
Abstract
Back To Researches Page