Document Details

Document Type : Article In Journal 
Document Title :
Free fatty acids (FFAs) – induced PKC and NFB activation, two key events in two different models for insulin resistance, the skeletal muscle and liver
Free fatty acids (FFAs) – induced PKC and NFB activation, two key events in two different models for insulin resistance, the skeletal muscle and liver
 
Subject : Free fatty acids (FFAs) – induced PKC and NFB activation, two key events in two different models for insulin resistance, the skeletal muscle and liver 
Document Language : English 
Abstract : We examined the effects of FFAs on insulin signaling in both muscle and liver. In the muscle, the glucose uptake showed a 50% increase over basal in control cells treated with insulin. However, cells treated with either oleate or palmitate showed a statistically significant reduction in insulin stimulated glucose uptake (p<0.05). The PKB was reduced by 26% in cells treated with oleate and > 50% (p<0.05) reduction in cells treated with palmitate. The oleate treated myocytes showed a significant increase in the phosphorylation of PKC alpha, beta, delta and epsilon isoforms. PKC inhibition with Bis-I (2 mM) was capable of reversing the oleate induced insulin resistance in myocytes by improving the glucose uptake and the PKB phosphorylation status. FFA treatment in myocytes led to the activation of NFkB as shown from the expression of IKK in the kinases screen. In contrast there was no significant changes in cultured primary hamster hepatocytes for the insulin receptor tyrosine phosphorylation or mass even in the presence of high insulin. The Phosphosite showed an increased phosphorylation of PKC- related kinase 1 and 2 with the activation of the Ras-MAPK pathway and a reduction in the expression level of IB in the fructose fed hamster. The data show a direct desensitization of muscle cells following FFA exposure but no observable changes in hepatic insulin signaling, suggesting that FFA-induced insulin resistance is exerted through different mechanisms. And it points out the importance of PKC and NFB activation following direct treatment with FFA in the two models. 
ISSN : 0 
Journal Name : Journal of Diabetes and Vascular Disease Research 
Volume : 3 
Issue Number : 2 
Publishing Year : 2006 AH
1427 AD 
Article Type : Article 
Added Date : Monday, June 15, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
أمينةAminaInvestigatorDoctorate 
GamilaGamilaResearcherDoctorate 
ديناDinaResearcherDoctorate 
FantusFantusResearcherDoctorate 
AdeliAdeliResearcherDoctorate 

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