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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Massively parallel sequencing of forensic STRs: considerations of the DNA commission of the International Society for Forensic Genetics (ISFG) on minimal nomenclature requirements
Massively parallel sequencing of forensic STRs: considerations of the DNA commission of the International Society for Forensic Genetics (ISFG) on minimal nomenclature requirements
 
لغة الوثيقة : الانجليزية 
المستخلص : The DNA Commission of the International Society for Forensic Genetics (ISFG) is reviewing factors that need to be considered ahead of the adoption by the forensic community of short tandem repeat (STR) genotyping by massively parallel sequencing (MPS) technologies. MPS produces sequence data that provide a precise description of the repeat allele structure of a STR marker and variants that may reside in the flanking areas of the repeat region. When a STR contains a complex arrangement of repeat motifs, the level of genetic polymorphism revealed by the sequence data can increase substantially. As repeat structures can be complex and include substitutions, insertions, deletions, variable tandem repeat arrangements of multiple nucleotide motifs, and flanking region SNPs, established capillary electrophoresis (CE) allele descriptions must be supplemented by a new system of STR allele nomenclature, which retains backward compatibility with the CE data that currently populate national DNA databases and that will continue to be produced for the coming years. Thus, there is a pressing need to produce a standardized framework for describing complex sequences that enable comparison with currently used repeat allele nomenclature derived from conventional CE systems. It is important to discern three levels of information in hierarchical order (i) the sequence, (ii) the alignment, and (iii) the nomenclature of STR sequence data. We propose a sequence (text) string format the minimal requirement of data storage that laboratories should follow when adopting MPS of STRs. We further discuss the variant annotation and sequence comparison framework necessary to maintain compatibility among established and future data. This system must be easy to use and interpret by the DNA specialist 
ردمد : 1872-4973 
اسم الدورية : Forensic Science International: Genetics 
المجلد : 22 
العدد : 1 
سنة النشر : 1437 هـ
2016 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Tuesday, July 11, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Walther ParsonParson, Walther باحثدكتوراه 
David BallardBallard, David باحثدكتوراه 
Bruce BudowleBudowle, Bruce باحثدكتوراه 
John M. ButlerButler, John M. باحثدكتوراه 
Katherine B. GettingsGettings, Katherine B. باحثدكتوراه 
Peter GillGill, Peter باحثدكتوراه 
Leonor GusmãoGusmão, Leonor باحثدكتوراه 
Douglas R. HaresHares, Douglas R. باحثدكتوراه 
Jodi A. IrwinIrwin, Jodi A. باحثدكتوراه 
Jonathan L. KingKing, Jonathan L. باحثدكتوراه 
Peter de Knijffde Knijff, Peter باحثدكتوراه 
Niels MorlingMorling, Niels باحثدكتوراه 
Mechthild PrinzPrinz, Mechthild باحثدكتوراه 
Peter M. SchneiderSchneider, Peter M. باحثدكتوراه 
Christophe Van NesteVan Neste, Christophe باحثدكتوراه 
Sascha WilluweitWilluweit, Sascha باحثدكتوراه 
Christopher PhillipsPhillips, Christopher باحثدكتوراه 

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اسم الملفالنوعالوصف
 41942.pdf pdf 

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